mastercycler nexus gradient thermal cycler (Eppendorf AG)

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Eppendorf AG mastercycler nexus gradient thermal cycler
Mastercycler Nexus Gradient Thermal Cycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 97/100, based on 1304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mastercycler+gradient/Mastercycler+nexus/pm42891932-38-7-6
Average 97 stars, based on 1304 article reviews

mastercycler nexus gradient thermal cycler - by Bioz Stars, 2026-11

97/100 stars

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Polymerase Chain Reaction:

Article Title: Purification and Transcriptomic Characterization of Hypertrophied Hepatic Stellate Cells From CDAHFD Mouse Liver
Article Snippet: .. Standard PCR 0.15 units of Taq polymerase (AmpliTaq Gold, Life Technologies) and 20 pmol/μL of the forward and reverse primers (IDT Technologies) was run in a Mastercycler gradient (Eppendorf) at 94°C for 3 min, followed by 35 cycles of 94°C for 45 s, 56°C for 60 s, 72°C for 90 s and a final cycle of 72°C for 10 min. ..

Article Title: Short-term nitrogen enrichment alters microbial phosphorous limitation in Pinus taiwanensis forest soils.
Article Snippet: For each soil sample, a 10-digit barcode sequence was incorporated at the 5ʹ end of the forward and reverse primers (Allwegene Company, Beijing, China). .. QPCR was conducted on a Mastercycler Gradient (Eppendorf, Hamburg, Germany) using 25 μl reaction volumes, comprising 12.5 μl 2× Taq PCR MasterMix, 3 μl bovine serum albumin (2 ng μl -1 ), 2 μl primers (5 μM), 2 μl template DNA, and 5.5 μl ddH2O. ..

Article Title: Platform-Specific Immunoassay Development and Orthogonal Characterization for Improved Host Cell Protein Control in Vero Cell-Derived Vaccines.
Article Snippet: Samples were tested with quantitative Western blot analysis on Wes or Jess systems (Protein Simple) employing the appropriate selection of size‐based assay kits (Gillespie et al. 2023). .. Briefly, samples and HCP standards were denatured with a master mix solution in a Thermo Scientific Armadillo PCR 96‐well plate for 5 min at 95°C using a Mastercycler Gradient (Eppendorf). ..

Article Title: MITF programs macrophage iron homeostasis to drive gut-liver inflammatory axis in early life.
Article Snippet: For each sample, 8-digit barcode sequence was added to the 5′ end of the forward and reverse primers (provided by Allwegene Company, Beijing). .. The PCR amplification was carried out on a Mastercycler Gradient (Eppendorf, Germany) using 25 μL reaction volumes containing 12.5 μL 2× Taq PCR MasterMix, 3 μL BSA (2 ng/μL), 1 μL Forward Primer (5 μM), 1 μL Reverse Primer (5 μM), 2 μL template DNA, and 5.5 μL double distilled H2O. ..

Article Title: Protein levels alter yak rumen microbiota profiles, meat properties, and longissimus dorsi metabolites
Article Snippet: For each sample, 8-digit barcode sequence was added to the 5′ end of the forward and reverse primers (Allwegene). .. The polymerase chain reaction (PCR) was carried out on a Mastercycler Gradient (Eppendorf) using 25 μL reaction volumes, containing 12.5 μL 2× Taq PCR MasterMix (Vazyme Biotech), 3 μL BSA (2 ng/μL), 1 μL Forward Primer (5 μM), 1 μL Reverse Primer (5 μM), 2 μL template DNA, and 5.5 μL ddH2O. ..

Article Title: Purification and Transcriptomic Characterization of Hypertrophied Hepatic Stellate Cells From <scp>CDAHFD</scp> Mouse Liver
Article Snippet: .. Standard PCR 0.15 units of Taq polymerase (AmpliTaq Gold, Life Technologies) and 20 pmol/μL of the forward and reverse primers (IDT Technologies) was run in a Mastercycler gradient (Eppendorf) at 94°C for 3 min, followed by 35 cycles of 94°C for 45 s, 56°C for 60 s, 72°C for 90 s and a final cycle of 72°C for 10 min. ..

Bioprocessing:

Amplification:

other:

Article Title: Carbon dots alleviate salinity stress and promote growth in tomato ( Solanum lycopersicum )
Article Snippet: The subsequent thermal protocol was executed using a thermal cycler (Eppendorf, Mastercycler gradient, Germany): incubation at 25°C for 10 min, followed by 47°C for 60 min, cessation of the reaction by heating to 85°C for 5 min, and subsequent cooling on ice (at 4°C).

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